Serratia peptidase is an enzyme that finds a wide range of applications in Pharmaceutical industry, health care and Medicine etc. SERRATIA MARSCENES was isolated from oil rich soil and silk worm source and screened for the production of Serratia peptidase. Different nitrogen and carbon source like soya peptone, normal peptone, ammonium nitrate and ammonium sulphate, sugar, rice flour, mannitol, ragi flour were used in the production media. The maximum production was in the ammonium sulphate, sugar for MTCC culture and ammonium sulphate, rice flour for AZRM 1 culture containing media. The enzymes were purified to homogeneity by ammonium sulphate precipitation, acetone precipitation and ion exchange chromatography. Characterization studies showed optimum pH 7 and optimum temperature was 370C. The enzyme activity was increased by Magnesium chloride and inhibited by mercuric chloride. The molecular weight of the enzyme as determined by SDS-PAGE was found to be 50-55KDa. The anticoagulant property of the enzyme was also studied which gave delayed clotting time for blood samples. Preparations of proteolytic enzymes have been shown to be useful in cancer, digestion support, fibrocystic breast disease, food allergies, hardening of the arteries (atherosclerosis), hepatitis C, herpes zoster (shingles), inflammation, sports injuries and trauma, pancreatic insufficiency, multiple sclerosis, rheumatoid arthritis and other autoimmune disorders, sinusitis, asthma, bronchitis, and chronic obstructive pulmonary disease.
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